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文献和实验CsCl soln. 11) Centrifuge at 40 K for 48 hrs in an SW-55 (or comparable) rotor. Injection DNA Preparation (cont) 12) Pull lower (plasmid) band using a 22.5 gauge needle and a 1 ml syringe. 13) Extract EtBr with water saturated butanol or water
Marcantonio Lab Protocol Manual
when handling and be sure to dispose of properly. 2) Transfer the solution to a Beckman Quickseal ultracentrifuge tube. This can be done using a syringe and needle or by using the Beckman funnel. Top off the tube, if necessary, using CsCl/TE solution (1 g/1 ml
BAC DNA Purification For Injection
leaking). Wash the column three times with 10 ml of the injection buffer (autoclave-sterilized 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl). This takes 2-3 hours. Add 5 µl of any Bromophenol Blue-containing DNA loading buffer to the 0.1 ml
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