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文献和实验A simple method for DNA extraction from formalin-fixed, paraffin embedded tissue blocks
microtube (1.5ml and 0.5 ml) and pipette tips were autoclaved as well as sterile distilled water used. After delivering tissues from Pathology department, tissues were carved with a surgical blade into 1mm pieces and between 25 mg to 75 mg
Natural History Museum Protocol for EST sequencing from lamb
PCR reaction (32µl) into a 1.5ml microtube, vortex briefly to mix and then add 1ml of "Wizard Resin" and vortex briefly, 3 times over a 1 minute period remove the plunger from a 3ml, luer l℃k syringe and attach the barrel to a clean "Wizard
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
" (see below) to the oil-free PCR reaction (32µl) into a 1.5ml microtube, vortex briefly to mix and then add 1ml of "Wizard Resin" and vortex briefly, 3 times over a 1 minute period remove the plunger from a 3ml, luer lock syringe and attach the barrel to a clean
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