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文献和实验Alkaline Southern Blotting Procedure
alone. 8.Invert gel, place into .25M HCl for 7 min. 9.Rinse briefly in dH2O. 10.Soak in 0.3M NaOH,0.3M NaCl for 10 min. 11.Set up a transfer tray (9 X 12 inch glass baking dish) by pouring transfer solution in the tray (about 1000 ml) and placing a glass
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Alkaline Southern Blotting Procedure
and an additional 1 sec on gel alone. Invert gel, place into .25M HCl for 7 min. Rinse briefly in dH2O. Soak in 0.3M NaOH,0.3M NaCl for 10 min. Set up a transfer tray (9 X 12 inch glass baking dish) by pouring transfer solution in the tray (about 1000 ml
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