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上海希言科学仪器有限公司
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文献和实验alpha primers are used as an equimolar pool Reaction Mixture 10 x labeling buffer - 3.0 ul 10 x reaction solution - 3.0 ul primer - 60~200 pmol Taq DNA polymerase 3.0 ul (3 u) ddH2O to 30 µl
conjugate mount in 20 µl of anti-fade solution containing propidium iodide (30 ng/ml) and DAPI (30 ng/ml) to counterstain the chromosomes cover with a cover slip, remove excess mountant and observe
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorp
after adding SDS. Denature probe by heating for 2 min at 100 o C, and spin at 14,000 RPM for 15-20 min. Place the entire probe volume on the array under a the appropriately sized glass cover slip. Hybridize at 65o C for 14 to 18 hours
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