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文献和实验Internal quality control (IQC) is a valuable technique to ensure that the results produced from any assay are reliable and reproducible. Here I describe how to set up an IQC program for a new clinical assay based on IQC samples
Charting Methods for Internal Quality Control
. This assay is available in kit form. Constant evaluation of the use of the kit is part of what is called internal quality control (IQC). Figure 1 shows an overview of the ELISA scheme described in this chapter. The details of the procedure, which involves
Use of Internal and External Standards or Reference RNAs for
under investigation are compared from sample to sample using an internal control to normalize for differences in sample concentration and loading. Ideally, the internal control should be a gene expressed at a constant level across the sample set. In absolute
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