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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
protocol has been optimized using Jurkat cells (human T-cell leukemia line) at a concentration of 1 × 106 cells/mL. Use of other cell types or other cell concentrations may require optimization of staining. If another staining reaction is to be performed
Northern Blotting方法(Howell Lab)
5 ml of scintillation fluid and count. % incorporation = (cpm of washed/cpm of unwashed) x 100. Anything over 10% is usually usable. Using the kit, it's usually 40%. The specific activity (S.A.) of the probe is also important. S
It is good practice to check both the integrity (agarose gel) and the concentration (absorbance) of the standard before use. Fluor Buffer Hoechst 33258 solution* (from kit) 25 µl TNE Buffer** (from kit) 10 ml * Hoechst 33258
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