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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
expression in several tumor cell lines, activated human lymphocytes and chick chondrocytes. Below , HL-60 cells labeled for cyclin D1 or cyclin D2/D3 with 80% EtOH treatment at �20°C. References Gong, J., Bhatia, U., Traganos, F
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
to detect cyclin D1 expression in several tumor cell lines, activated human lymphocytes and chick chondrocytes. Below, HL-60 cells labeled for cyclin D1 or cyclin D2/D3 with 80% EtOH treatment at –20℃. References Gong, J., Bhatia, U
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
antibody against surface marker of interest 0.1% paraformaldehyde in PBS 70% EtOH in glycerol (stored at 4°C) staining buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 m g/ml solution with 100 U/ml DNase-free
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