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文献和实验Double-strand breaks (DSBs) in genomic DNA are induced by ionizing radiation or radiomimetic drugs, but they also occur spontaneously during the cell cycle at quite significant frequencies. In vertebrate cells, nonhomologous DNA end joining
【求助】allele specific expression (ASE) assay
youyoubantang 想请教各位大侠,等位基因特异性表达分析:allele specific expression (ASE) assay 到底是什么,他的原理及操作步骤是怎样的,特别是原理,一直搞不明白 还有就是他怎样验证cis-eQTL 的真实性 shilei5794749 DOI 10.1016/j.cell.2010.09.049 看这篇文章!讲 long-noncoding RNAs 中 Jpx 调节
medium through the flow chamber, again using a 10 ml syringe. If large bubbles remain, raise the outflow end of the chamber to a 45° angle (Fig. 8). 9) Cap all of the 4-way stopcock connections and close them off. Move the flow chamber
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