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文献和实验C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice
Through directed differentiation of embryonic stem cells, it has been demonstrated that mesodermal lineages in the mammalian heart (smooth muscle, endothelial, and cardiac) develop from a common, multipotent cardiovascular precursor (Dev Biol
a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
7.6, and 1.37 MNaCl. Store at room temperature. 2. TBS with Tween (TBS-T) (10×): prepare a TBS solution (X1)and add 0.1% (v/v) Tween-20. 3. 5% (w/v) Non-fat dry milk in TBS-T. 4. Primary antibody: APC (Ab-1) antibody from OncogeneResearch.
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