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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x volume of filtrate) to each well. Seal with plastic sealing tape and mix. Incubate
E-Z 96® Viral RNA Protocol with Centrifugation
into the each well of the HiBind® RNA plate . Seal the plate with a new sealing film. Centrifuge at 5500 x g for 5 minutes at room temperature. 11. Place HiBind® RNA plate on top of another clean 2ml 96-well plate (Not supplied). Remove the sealing film and add 500 ul Wash
conditions are the same for all washes unless otherwise stated. Wash the Ni-NTA resin three times each with 30 µL of 100 mM EDTA (pH 8.0) to remove the bound Ni2+ metal. Wash the column three times each with 30 µL of H2O. Wash the column three times
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