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文献和实验E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
. It is recommended to homogenize the sample with rotor-stator homogenizers since it normally produce better yield. 2. Spin at 5,000 x g for 10 min at room temperature. Transfer the supernatant into a new tube and add an equal volume (2ml or 4ml) 70% Ethanol
suspension for 6 x 10 second bursts (with 10 second intervals between each burst) using a Misonix XL 2020 sonicator fitted with a microtip probe and set at power setting 5 (Figure 4 Left). Check the sonicated nuclei under a phase contrast microscope
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
. It is best to use a rotor–stator homogenizer to homogenize cells for 30 s. If not, pass the lysate at least 5 times through a needle fitted to an RNase-free syringe. 4. Add 1 volume (usually 350 µl or 600 µl) 70% ethanol, mix
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