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文献和实验at 70-80% confluency or as advised on ECACC's cell culture data sheet.Do not allow media to go out of date. Shelf life is only 6 weeks at +4oC once glutamine and serum is added.Avoid water baths from becoming dirty by using Sigma Clean (Prod. No. S5525
Subculture of Suspension Cell Lines
are obtained within 4 weeks. Mycoplasma colonies observed on agar plates have a `fried egg? appearance (see Figure 14 ). Materials 70% ethanol in water (Prod. No. R8382 ) Mycoplasma Pig Agar plates (in 5cm petri dishes) Mycoplasma Pig Agar
Subculture of Suspension Cell Lines
at 70-80% confluency or as advised on ECACC's cell culture data sheet.Do not allow media to go out of date. Shelf life is only 6 weeks at +4ºC once glutamine and serum is added.Avoid water baths from becoming dirty by using Sigma Clean (Prod. No. S5525
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