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文献和实验2) to room temperature.c.Warm pre-poured LB plates with 50 µg/ml ampicillin at 37℃ for 30 minutes.d.Spread 40 µl of 40 mg/ml X-gal (in foil-coated box in -20℃ freezer) on each LB plate and incubate at 37℃ until ready for use.e.Thaw 1 vial of One Shot cells
at 37℃ for 30 minutes. d.Spread 40 µl of 40 mg/ml X-gal (in foil-coated box in -20℃ freezer) on each LB plate and incubate at 37℃ until ready for use. e.Thaw 1 vial of One Shot cells for every three transformations. 3.Set
:加入溶液,在4oC颠转10min,4oC静置10min沉淀,700rpm离心1min,除去上清。 洗涤溶液:a.low salt wash buffer-one wash b.highsalt wash buffer-one wash c.LiCl wash buffer-one wash d.TE buffer-two wash 15、清洗完毕后,开始洗脱。洗脱液的配方:100ul10%SDS,100ul1MNaHCO3,800
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