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文献和实验Heat at 94°C for 10 min. Precipitate the DNA using 0.1 x volume 3 M sodium acetate, and 2.5 x volume 100% ethanol. Resuspend the DNA pellet in 40 µl water. To 12.2 µl DNA from #10 above, add the following re-amplification
% sucrose from the top of the gel. Apply 10 ul (about 0.15 ug DNA for rice, or about 0.45 ug DNA for egg plant) sample on a 1-D gel. Run electrophoresis at 130 Volts (120 - 140 Volts) for 40 hrs (until the center of BPB reaches 40 cm and that of XC
1. Materials Protease inhibitor cocktail (Boehringer Mannheim) 2X SDS sample buffer (Novex) Pre-casted gels (e.g. Novex) Molecular weight protein standard (e.g. MultiMark Multi-Colored Standard, Novex) 25X Tris
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