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文献和实验Western Blotting Using the SemiPhor Semi-Dry Transfer Unit
to a suitable power supply. Turn the power supply to zero before turning on. Turn on the power supply and set to approximately 0.8 mA/cm2 of gel. 8 mm x 7 mm mini-gels may be run at 100 mA constant current. Larger gels must be limited to 0.8 mA/cm2 to avoid
Western Blotting Using the SemiPhor Semi-Dry Transfer Unit
the unit to a suitable power supply. Turn the power supply to zero before turning on. Turn on the power supply and set to approximately 0.8 mA/cm2 of gel. 8 mm x 7 mm mini-gels may be run at 100 mA constant current. Larger gels must be limited to 0.8 mA/cm
Semi-dry Transfer Apparatus Biorad, Cat# 170-3940, or equivalent Power Supply 0 - 100 VDC (adj. current to 1 Amp) Immobilon-Ptransfer membrane 0.45 µm pore size; cut to same size as gel (Millipore, Cat# IPVH304-FO, or equivalent) Filter Paper
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