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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
and dissolve the precipitate by gentle inversion. 10. Add 4 ml of 4 M Lithium Acetate and incubate on ice for 20 min. 11. Centrifuge at 1,000 X g for 10 min. 12. Transfer the supernatant to a fresh tube, add 16 ml of 100% Ethanol, and incubate
and allow it to settle by gravity. (DO NOT MIX)8. Gently wash the column 3X with 750 l 1X NETS. (Pipette down the side of the column without disrupting the cellulose.)9. Elute the mRNA into a fresh Eppendorf tube by pipetting 650 l of 65 !C 1X ETS directly
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