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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
现货
- 供应商:
鹿森生物
- 规格:
盒
Features
Fast procedure delivering high-quality total RNA in minutes
Ready-to-use RNA for high performance in any downstream application
Consistent RNA yields from small to large amounts of starting material
No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
High-throughput processing in 96-well format and automatable protocols
Product Details
RNeasy Kits are the gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured cell samples using silica membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect.
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文献和实验winniw214 小提的质粒(自己配的S1、S2和S3,不是试剂盒,但是师姐用过没问题的) 结果质粒浓度很低。菌液是37度,250rpm摇了11h的,应该够浓。 想问一下有没有什么改进的方法? 另外,关于加过S1、S2和S3之后的操作(剧烈混匀、颠倒……),我查过问过有好多不同的说法。不知道亲们平时怎么做的,哪种效果比较好? 谢谢 qijilaile s1,s2,s3混匀
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
(Promega);Genomyx LR 、 Genomyx SC、DNAThermal Cycler(Perkin Elmer)1.3 总RNA的制备按试剂盒提供的方法分别提取三种细胞的总 RNA,以 RNase-free DNase I(终浓度80 000 U/L)除去其中污染的 DNA,经甲醛变性凝胶电冰鉴定其完整性,并以紫外分光光度计检 测其纯度1.4 mRNA差异显示1.4.1 逆转录反应选择锚定引物[T7(dT12)AP(anchored prime rs,AP)],序
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