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- QIAGEN
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- 74134
- 2025年09月25日
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- 供应商：
鹿森生物
- 规格：
盒
Features
Efficient gDNA removal with unique gDNA Eliminator columns or plates (no need for DNase)
High-quality total RNA in minutes using fast and simple extraction protocols
Phenol-free RNA isolation
High-throughput processing in 96-well format
Ideal for sensitive applications such as real-time RT-PCR and RNA-seq
Product Details
RNeasy Plus Kits are next generation QIAGEN RNA extraction kits, combining high-quality RNA purification with effective gDNA removal with unique gDNA Eliminator columns or plates. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. The RNeasy Plus Micro Kit is specially designed for limited amounts of samples and isolates up to 45 µg pure total RNA The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (>200 nt) from a single extraction with efficient gDNA removal. Both kits can be automated on the QIAcube Connect and samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system (available separately). The RNeasy Plus 96 Kit is for high-throughput purification of total RNA from cultured cells grown in 96-well plates.
In partnership with My Green Lab, we've also assessed the environmental impact of the RNeasy Mini Kit (50/250) and the QIAwave RNA Plus Mini Kit (50/250). My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:
Manufacturing
Responsible chemical management
Sustainable content within products and packaging materials
Disposal of the packaging at the end of life
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文献和实验适合从动物细胞、动物组织或酵母菌中同步纯化总RNA 和基因组DNA纯化的总RNA ，适合用于Northern Blot、RT-PCR、体外翻译、Primer Extension、S1 核酸酶作图、RNase 保护测定、制备mRNA 和构建cDNA 文库等各种RNA 分子生物学实验。纯化的基因组DNA 适用于PCR、Southern Blot、RAPD、AFLP、RFLP 等分子生物学实验。一、试剂盒组成、贮存、稳定性以每次从1×107 动物细胞、100mg 动物组织或5×107 酵母菌中
酶消化。(5) RN28 EASYspin Plus组织/细胞RNA快速提取试剂盒。这款等于是Qiagen的74134完全一样，是目前世界上最先进的试剂盒。15分钟提取，不用苯酚，氯仿，也不残留DNA，不用DNA酶消化直接可以做荧光定量PCR，唯一缺点，就是好货不算便宜。 总结：首先推荐就是RN03 RNApure 超纯总RNA快速提取试剂盒或者RN28 EASYspin Plus组织/细胞RNA快速提取试剂盒这两款产品。其它的随机应变。根据客户需要来，客户觉得RN28贵，就推RN03,还觉得贵，就推RN01
winniw214 小提的质粒（自己配的S1、S2和S3，不是试剂盒，但是师姐用过没问题的） 结果质粒浓度很低。菌液是37度，250rpm摇了11h的，应该够浓。 想问一下有没有什么改进的方法？ 另外，关于加过S1、S2和S3之后的操作（剧烈混匀、颠倒……），我查过问过有好多不同的说法。不知道亲们平时怎么做的，哪种效果比较好？ 谢谢 qijilaile s1,s2,s3混匀
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