StemSpan™ SFEM

StemSpan™ Serum-Free Expansion Medium (SFEM) has been developed for the in vitro culture and expansion of human hematopoietic cells. This medium contains pre-tested bovine serum albumin, insulin and transferrin, and supplements in Iscove’s MDM. Recombinant hematopoietic growth factors, required for the optimal growth and expansion of hematopoietic cells, have not been added to StemSpan™ SFEM. This allows users the flexibility to prepare medium that meets their requirements.
In addition to the culture and expansion of human hematopoietic cells, StemSpan™ SFEM has been used for the culture and expansion of hematopoietic stem and progenitor cells from other species, including mouse, non-human primate and dog. StemSpan™ SFEM has also been used for the generation, culture and assay of human and mouse dendritic cells, and for the culture of various other hematopoietic and non-hematopoietic cell types. In most applications, addition of specific hematopoietic growth factors, cytokines and/or other compounds is required for optimal growth.