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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品包装
- 保质期:
一年
- 英文名:
SDS-polyacrylamide gel electrophoresis (SDS-PAGE
- 库存:
大量
- 供应商:
QIYBO
- 规格:
10 ml (5 x 2 ml)
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
, a pair of frogs were mated (see Grainger Lab X. tropicalis Mating Protocol for details) and the resultant tadpoles were used in the following karyotyping protocol. 2. Place 10 Nieuwkoop & Faber Stage 26-34 tadpoles into a dish of deionized, distilled
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
by inverting the tube a few times. Incubate 5 minutes at room temperature. Invert the tube several times during incubation. NOTE: ERL Buffer is supplied as a 10x concentrate and must be diluted with ddH2 O according to bottle label. 2. Centrifuge
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