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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD45RA
- 亚型:
Mouse IgG2a
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PE
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:HI100
- 抗体英文名:
CD45RA Antibody-PE;流式抗体
- 抗体名:
CD45RA Antibody-PE;流式抗体
- 规格:
25/100 tests
克隆类型 :Mouse Monoclonal Antibody;Clone number:HI100
货 号 :HT1610953
规 格 :25/100 tests;
亚 型 :
Mouse IgG2a
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naive T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used from 20 µL to 5 µL per 100 µL of peripheral blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PE(其他荧光标记请咨询我们)
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文献和实验Phycoerythrin conjugation protocol
Centricon 30 and Centriprep 30 centrifugal concentrators (Amicon) * Room temperature is about 22 °C Method 1. Preparation of PE 2. SPDP modification of PE 3. SMCC modification of antibody 4. DTT
Sequencing off Cosmid, BAC, PAC, and Fosmid Templates with ABI Big Dye Terminators
conditions obtained from Cheryl Heiner rather than the FS cycle sequencing conditions, and by increasing the number of cycles to 100 overnight. Typically we are using 18-22 mers as primers where no more than 65% identity (i.e. Note
实验简介13 色淋巴亚群免疫功能检测,利用高度集成,卓越检测性能的 CytoFLEX 对外周血中高度异质的 T 淋巴细胞亚群进行分析,10 色 Duraclone IM T 细胞试剂盒 (CD45RA-FITC,CD197-PE,CD28-ECD,CD279-PC5.5,CD27-PC7,CD4-APC,CD8-Alexa-Fluor 700,CD3-APC-AF750,CD57-Pacific Blue,CD45-Krome Orange) 为贝克曼最新用于淋巴亚群检测的干粉试剂。加上
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