- ￥6200
- GeneTex
- 美国
- GTX33912-13
- 2025年07月14日
- ICC/IF
- Human
- Human, Mouse, Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Bovine brain tubulin.
- 亚型：
IgG2
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
ATTO-488
- 适应物种：
Human, Mouse, Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Bovine
- 目录编号：
GTX33912-13
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Human
- 应用范围：
ICC/IF
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Recognizes mouse, bovine and human α-tubulin.
- 抗体英文名：
alpha Tubulin antibody [F2C] (ATTO-488)
- 抗体名：
alpha Tubulin 抗体 [F2C] (ATTO-488)
- 规格：
100 μg
Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C) (ATTO 488). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alphaTubulin, mAb (rec.) (F2C) (ATTO 488) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
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文献和实验Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
immunofluorescence. Block in AbDil for 10'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst. A rapid rinse will be sufficient. Drain, mount and seal. COMMENT
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
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