手机验证
充足
杭州昊鑫生物
15.6-1,000pg/mL
双抗夹心
Mus musculus (Mouse,小鼠)
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
48T
本试剂盒用于检测白介素2(IL2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的白介素2(IL2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 79-104 | 97 |
EDTA plasma(n=5) | 92-101 | 97 |
heparin plasma(n=5) | 78-102 | 92 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的白介素2(IL2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素2(IL2)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-104% | 85-104% | 93-101% | 79-89% |
EDTA plasma(n=5) | 78-101% | 95-103% | 80-97% | 80-89% |
heparin plasma(n=5) | 83-101% | 91-105% | 78-89% | 84-105% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将白介素2(IL2)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的白介素2(IL2)与连接于固相载体上的抗体结合,然后加入生物素化的白介素2(IL2)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素2(IL2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
杂志 | 参考文献 |
Journal of Medicinal Plants Research | Evaluation of water plantain (Alisma canaliculatum A. Br. et Bouche) and mistletoe (Viscum album L.) effects on broiler growth performance, meat composition and serum biochemical parameters [AcadeMic: source] |
Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration [PubMed: 22796416] |
Nanomedicine | Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132] |
Food and Chemical Toxicology | Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta [Pubmed:25662032] |
Pharmaceutical Research | Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats [Openview: 6Ba34F298130121B36Fc3E227Ed416F4] |
PLoS Neglected Tropical Diseases | Efficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in Mice [PubMed: 26352932] |
Int J Clin Exp Med | Berberine alleviates preeclampsia possibly by regulating the expression of interleukin-2/interleukin-10 and Bcl-2/Bax [PubMed: 26629148] |
Clin Oral Investig | Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? [PubMed: 26556576] |
veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ] |
Pharmaceutical Biology | Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats [PubMed: 25858514] |
Immunologic Research | Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE10 and is crucial for liver transplant tolerance [PubMed: 25982596] |
J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
удк | Циток [Cgi-Bin: Irbis_Nbuv] |
Biomark Med | Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma [PubMed: 26609634] |
Environmental Science and Pollution Research | Ameliorative effect of flaxseed oil against thiacloprid-induced toxicity in rats: hematological, biochemical, and histopathological study [Pubmed:26957428] |
Advances in Experimental Medicine and Biology | Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193] |
Research in Veterinary Science | Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens [Pubmed:27473977] |
Biomedicine & Pharmacotherapy | Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate [Pubmed:27261592] |
Int J Pharm Bio Sci | International Journal of Pharma and Bio Sciences ISSN [cms:4987] |
Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790] |
United States Patent Application | HERPES VIRUS VACCINES AND TREATMENTS [y2016:0067330.html] |
BMC Veterinary Research | Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600] |
Vaccine. | Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. [pubmed:28049588] |
Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494] |
Physiological Research | Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947] |
Journal of Virology | Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:] |
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杂志 | 参考文献 |
Journal of Medicinal Plants Research | Evaluation of water plantain (Alisma canaliculatum A. Br. et Bouche) and mistletoe (Viscum album L.) effects on broiler growth performance, meat composition and serum biochemical parameters [AcadeMic: source] |
Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration [PubMed: 22796416] |
Nanomedicine | Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132] |
Food and Chemical Toxicology | Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta [Pubmed:25662032] |
Pharmaceutical Research | Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats [Openview: 6Ba34F298130121B36Fc3E227Ed416F4] |
PLoS Neglected Tropical Diseases | Efficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in Mice [PubMed: 26352932] |
Int J Clin Exp Med | Berberine alleviates preeclampsia possibly by regulating the expression of interleukin-2/interleukin-10 and Bcl-2/Bax [PubMed: 26629148] |
Clin Oral Investig | Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? [PubMed: 26556576] |
veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ] |
Pharmaceutical Biology | Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats [PubMed: 25858514] |
Immunologic Research | Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE10 and is crucial for liver transplant tolerance [PubMed: 25982596] |
J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
удк | Циток [Cgi-Bin: Irbis_Nbuv] |
Biomark Med | Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma [PubMed: 26609634] |
Environmental Science and Pollution Research | Ameliorative effect of flaxseed oil against thiacloprid-induced toxicity in rats: hematological, biochemical, and histopathological study [Pubmed:26957428] |
Advances in Experimental Medicine and Biology | Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193] |
Research in Veterinary Science | Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens [Pubmed:27473977] |
Biomedicine & Pharmacotherapy | Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate [Pubmed:27261592] |
Int J Pharm Bio Sci | International Journal of Pharma and Bio Sciences ISSN [cms:4987] |
Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790] |
United States Patent Application | HERPES VIRUS VACCINES AND TREATMENTS [y2016:0067330.html] |
BMC Veterinary Research | Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600] |
Vaccine. | Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. [pubmed:28049588] |
Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494] |
Physiological Research | Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947] |
Journal of Virology | Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:] |