白介素4(IL4)检测试剂盒(酶联免疫吸附试验法)

白介素4(IL4)检测试剂盒(酶联免疫吸附试验法)

本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清或其它相关生物液体中 IL4 含量。

优尔生一级代理：杭州昊鑫生物
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优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,000种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真核（酵母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。
 

• 编号SEA077Hu
• 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
• 实验方法双抗夹心
• 反应时长3h
• 检测范围15.6-1,000pg/mL
• 灵敏度最小可检测剂量小于等于5.9pg/mL.
• 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• 下载英文说明书   中文说明书
• 规格48T96T 96T*5 96T*10 96T*100
• 价格¥ 2092

产品包装图模拟

 

特异性

本试剂盒用于检测白介素4(IL4)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素4(IL4)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本	回收率范围(%)	平均回收率(%)
serum(n=5)	95-104	99
EDTA plasma(n=5)	96-105	101
heparin plasma(n=5)	95-103	98

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100 
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10% 
批间差： CV<12% 

线性

在定值血清及血浆样本内加入适量的白介素4(IL4)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素4(IL4)含量的测定值与理论值的比率。

样本	1:2	1:4	1:8	1:16
serum(n=5)	78-96%	99-105%	98-105%	93-101%
EDTA plasma(n=5)	81-101%	80-91%	86-97%	86-101%
heparin plasma(n=5)	84-103%	80-105%	92-104%	96-104%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将白介素4(IL4)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的白介素4(IL4)与连接于固相载体上的抗体结合，然后加入生物素化的白介素4(IL4)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素4(IL4)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

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