结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)

结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)

优尔生一级代理：杭州昊鑫生物
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优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,000种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真核（酵母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。

• 编号SEA817Hu
• 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
• 实验方法双抗夹心
• 反应时长3h
• 检测范围0.625-40ng/mL
• 灵敏度最小可检测剂量小于等于0.307ng/mL.
• 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• 下载英文说明书 中文说明书
• 规格48T96T 96T*5 96T*10 96T*100

产品包装图（模拟）

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将结合珠蛋白(Hpt)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的结合珠蛋白(Hpt)与连接于固相载体上的抗体结合，然后加入生物素化的结合珠蛋白(Hpt)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的结合珠蛋白(Hpt)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

参考文献

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