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- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海冰晴生物科技有限公司
- 规格：
100 rxn
1）超高保真，通过大规模二代测序得出的出错率：2.8×10^(-7)；
2）可以扩增各种复杂模版；
3）扩增片段长，扩增基因组可达15kb，质粒DNA或噬菌体DNA可达18kb；
4）扩增速度快，节省75%以上的时间；
5）KAPA高保真热启动酶，含有2种以上buffer，分别用于常规模版和高难度模版。
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文献和实验Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed
one of the strands of the adapter to the fragment. The other is held on by base-pair binding to the other adapter strand. Thus the first step of the +1 PCR reaction is a 72 ˚C hold that allows the Taq polymerase to ligate the other strand. If you perform a Hot-Start
Single tube confirmation PCR protocol
until the PCR is started. Alternatively, a "hot start" can be performed by adding the Taq polymerase to the individual tubes after the PCR mixtures have been heated to 94°C. Hot start improves the PCR results but this step is labor intensive
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