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- 39.06 pg/mL - 2500 pg/mL
- 浙江杭州
- EK383
- 2026年04月10日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
39.06 pg/mL - 2500 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Rat
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
描述
| 商品名 |
Rat VEGF ELISA Kit (大鼠血管内皮生长因子 ELISA试剂盒) |
|---|---|
| 检测方法 |
双抗夹心法 |
| 精密度 |
板内变异系数：4.4% - 6.3%；板间变异系数：4.4% - 6.0% |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 |
20 μL |
| 灵敏度 |
3.86 pg/mL |
| 检测范围 |
39.06 pg/mL - 2500 pg/mL |
| 回收率 |
83% - 114% |
| 平均回收率 |
0.99 |
| 板式 |
96孔板，可拆 |
| 保存 |
试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 |
4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的VEGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
VEGFA 分子靶点信息概述
- 分子名：VEGFA, vascular endothelial growth factor A
- 基因家族：VEGF family
- 别名：VEGF-A; VPF
- 曾用名：VEGF
- 全称：vascular endothelial growth factor
VEGFA 分子靶点综述
血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。
大鼠 Rat Vegfa 分子靶点信息
- 分子名：Vegfa, vascular endothelial growth factor A
- 别称：
- vascular endothelial growth factor
- vascular permeability factor
- Vegf
- VEGF-A
- VEGF111
- VEGF164
- VPF
- 基因序列：NCBI_Gene: 83785
- 蛋白序列：
- UniprotKB: A0A0G2K6Z7
- UniprotKB: A0A0H2UHY5
- UniprotKB: B5DEK7
- UniprotKB: F1M6X1
- UniprotKB: F8WG85
- UniprotKB: G8JLS5
- UniprotKB: P16612
- UniprotKB: Q541S7
- UniprotKB: Q91ZE2
大鼠 Rat Vegfa 靶点分子功能（预测）
Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).
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