OptiDensity™样本密度分离液
60%碘克沙醇溶液
BasalMedia(R714)
OptiDensity™是即用型,无菌和超低内毒素含量的碘克沙醇溶液。适用于血液和组织中许多不同类型细胞的分离(如单核细胞、淋巴细胞、粒细胞、血小板、树突状细胞,megakaryotic祖细胞;肝非实质细胞,胰岛细胞,胰岛细胞;植物原生质体)以及细胞器的分离(如细胞核、线粒体、溶酶体、过氧化物酶体、胞内组分)和膜组分(如高尔基体膜、内质网、特定的质膜域)。从非活细胞的存活的分离。也适用于自生成的梯度。高效纯化病毒和病毒颗粒。也适用于分离蛋白质、脂蛋白、质粒DNA、核糖核蛋白等。
OptiDensity™分离液的主要成分是碘克沙醇(odixanol),是一种高亲水性非离子的物质,这种主要成分决定了OptiDensity™分离液是一种高密度、低粘度的溶液。而且,它的密度和渗透性成线形关系,在加入适当浓度的缓冲液或基本培养基,如蔗糖、Hepes、PRMI1640或DMEM后,OptiDensity™能形成连续的或不连续的等渗的密度梯度溶液,可将各种细胞、细胞器、脂蛋白分离开。经科研和临床实验证明,OptiDensity™和常规的分离液Ficoll、Percoll、蔗糖及氯化铯(CsCl)相比,OptiDensity™分离液不仅分离纯化效果好,而且对各种细胞、细胞器的生命活性无任何影响。
技术参数
组分
碘克沙醇(Iodixanol):60%(w/v)
水
理化特性
密度(Density): 1.320±0.001g/ml
渗透压(Osmolality): 170±15mOsm
内毒素含量(Endotoxins):< 0.1EU/ml
OptiDensity™Sample density separation
60% iodixanol solution
BasalMedia(R714)
OptiDensity™ is a ready-made, sterile and endotoxin-tested solution of iodixanol. Suitable for the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendriticcells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes,endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients.Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.
OptiDensity™ isolation liquid mainly ioxaglic alcohol (odixanol), is a highly hydrophilic non-ionic substances, this main component determines the separation of OptiDensity™ fluid is a high density, low viscosity of the solution.
Moreover,its density and permeability of a linear relationship, in appropriate concentrations of buffer or medium, such as sucrose, Hepes、PRMI1640 or DMEM, OptiDensity™ form a continuous or a discontinuousdensity gradient of isotonic solution, from a variety of cells, organelles, lipoprotein. Through research and clinical trials, compared to the conventional separation of fluid Ficoll, Percoll, sucrose and cesium chloride (CsCl),OptiDensity™ isolation liquid purification not only effective, but also for avariety of cells, cell life activity has no effect.
Technical parameter
Composition
Iodixanol: 60% (w/v) in water
Physical-chemical characteristics
Density: 1.320 ± 0.001 g/ml (20°C)
Osmolality: 170 ± 15 mOsm
Endotoxins: < 0.1EU/ml
图翎生物其他现货产品:
人/动物原代细胞
人/动物细胞系(株)
南美/澳洲 正品胎牛血清(抵制假货!)
基础培养基系列(RPMI1640、DMEM、Ham's F-12等)
减血清培养基系列
无血清培养基系列
组织、原代细胞和干细胞冻存液系列
病毒纯化分离液系列
各种细胞因子与抗体
AAV和CAV现货病毒系列
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