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- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
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- 英文名：
Thrombin Sepharose Beads
- 库存：
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- 供应商：
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- 规格：
1 ml
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文献和实验PREPARATION OF PROTEIN A SEPHAROSE CL 4B BEADS
Swelling and Storage1.Resuspend .2 gm of beads (= 1.0 ml swelled bead volume)in 40 ml of distilled water.Let swell for at least 2 hours.2.Wash beads twice in (10 ml each wash).Spin in clinical or table top fuge.Do not exceed 2K rpm.3.Resuspend
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
Purification of Antibodies Using Protein A-Sepharose and FPLC
at any one time. Since the antibody combining site is left free, protein A, when covalently coupled to stationary supports like agarose or Sepharose beads, provides an excellent reagent for isolating immune complexes or immunoglobulins from a crude solution
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