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- 抗体英文名：
pNPP One Component Microwell Substrate, Solution
- 抗体名：
pNPP One Component Microwell Substrate, Solution
- 规格：
100 mL
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文献和实验Lumigen HyPerBlu provides sensitive chemiluminescent detection of hydrogen peroxide
Abstract Lumigen HyPerBlu reagent is a one solution chemical substrate based on dioxetane chemiluminescent technology. With HyPerBlu reagent, a direct reaction with hydrogen peroxide produces an intense sustained luminescent signal making
DETECTION OF RECOMBINANT PROTEINS BY ELISA
2CO 3 slowly while stirring. Adjust pH to 8.6 and QS to 100 ml with H 2O. Substrate Solution (1 mg/ml paranitrophenyl phosphate PNPP) 100 mg capsule of PNPP (Sigma 104-100) 100 ml substrate buffer
should be titrated for optimal dilution) Add 100uL of second step antibody to wells and incubate for 1hr Repeat washing step Prepare substrate solution* Add 100uL of substrate to well and incubate at RT for 60min add stopping
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