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文献和实验as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
C. Avoid light exposure during preparation and storage. ABTS Stop Solution: Combine 50 ml dimethylformamide (DMF; Pierce, Cat. No. 20672) with 50 ml ddH2 O Add 20 g sodium dodecyl sulfate TMB
temperature for 5-20 minutes, depending on the rate of color development. Keep the time identical for subsequent comparisons of titer, and for hybridoma screening go the full 20 minutes. 10. Add 100 µL/well stop solution (0.5 M Oxalic Acid). 11. Read
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