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- 保存条件：
4度
- 保质期：
2018.3.23
- 库存：
1
- 规格：
1ml
Magne™ Protein A Beads and Protein G Beads beads use a novel attachment chemistry based on the HaloTag® technology to immobilize Protein A or Protein G. The HaloTag® protein is a multi-functional protein tag that forms a covalent bond with its ligand and is used for protein expression and purification, cell imaging, protein immobilization and protein-interaction analysis. Magne™ Protein A Beads and Magne™ Protein G Beads use recombinant Protein A from Staphylococcus and Protein G from Streptococci, respectively, fused with the HaloTag® protein. The Protein A-HaloTag® (M.Wt 71kDa) or Protein G-HaloTag® (M.Wt 58kDa) fusion protein is covalently attached in an oriented fashion to a magnetic cellulose bead that is activated using a HaloTag® ligand.
The Magne™ Protein A and Protein G Beads are not recommended for use in immunoprecipitation (IP) or co-IP applications.
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文献和实验PREPARATION OF PROTEIN A SEPHAROSE CL 4B BEADS
Swelling and Storage1.Resuspend .2 gm of beads (= 1.0 ml swelled bead volume)in 40 ml of distilled water.Let swell for at least 2 hours.2.Wash beads twice in (10 ml each wash).Spin in clinical or table top fuge.Do not exceed 2K rpm.3.Resuspend
Encapsulation of Cells in Agarose Beads
For the analysis of large DNA fragments using pulsed-field gel electrophoresis (PFGE), it is necessary to first embed the cells in agarose to prevent shearing of the DNA during protein extraction and restriction enzyme digestion. The speed
of 10 intensity levels and six colors could theoretically code 1 million nucleic acid or protein sequences. Imaging and spectroscopic measurements indicate that the quantum dot-tagged beads are highly uniform and reproducible, yielding bead
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