小鼠睾丸畸胎瘤细胞；F9说明书

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细胞名称 小鼠睾丸畸胎瘤细胞；F9
形态特性 上皮细胞
生长特性 贴壁生长
特征特性 在维生素A酸和联丁酰基cAMP刺激下，F9细胞分化成体壁内皮层。 分化的细胞合成血浆酶原活化因子，粘层蛋白和IV 型胶原质。 只有在经过维生素A酸处理后细胞上的cAMP才是活化的。 细胞中有三个拷贝β1粘合素基因。 检测表明肢骨发育畸形病毒(鼠痘)阴性。
培养条件 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)优质胎牛血清，10%
传代方法 消化3-5分钟。1:2。3天内可长满。
传代情况 PN5
冻存条件 完全培养基+8%DMSO
支原体检测 阴性
STR
同工酶
染色体
使用权限 A类
小鼠睾丸畸胎瘤细胞；F9操作步骤：
1）贴壁细胞传代：提前将培养基、PBS放入37℃水浴锅内预热，用75%酒精擦拭后再放入超净台内，吸除或倒掉细胞瓶内旧培养液，加少量PBS润洗细胞，加入适量胰酶，使胰酶的量能盖住细胞，37℃孵育，每隔2~3min显微镜下观察，待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶，加入新鲜培养基，晃动细胞瓶，终止胰酶作用，用吸管小心吹打贴壁的细胞，制成细胞悬液。控制吹打的力度，避免产生大量的气泡，将细胞悬液分别接种到另外的2~3个细胞瓶内，加入新鲜培养基，置37℃温箱培养，隔天观察贴壁生长情况。
2）悬浮细胞传代：将细胞悬液转移到无菌离心管内，1000rpm离心5min，弃去上清，加入新鲜的培养基，用吸管小心吹散沉淀，制成细胞悬液，将细胞悬液分别接种到另外的2~3个细胞瓶内，加入新鲜培养基，置37℃温箱培养。
LOC101864867 Cynomolgus monkey LOC101864867 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865220 Cynomolgus monkey LOC101865220 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865472 Cynomolgus monkey LOC101865472 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865491 Cynomolgus monkey LOC101865491 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865678 Cynomolgus monkey LOC101865678 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865719 Cynomolgus monkey LOC101865719 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865757 Cynomolgus monkey LOC101865757 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865772 Cynomolgus monkey LOC101865772 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865775 Cynomolgus monkey LOC101865775 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865810 Cynomolgus monkey LOC101865810 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865812 Cynomolgus monkey LOC101865812 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865828 Cynomolgus monkey LOC101865828 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865873 Cynomolgus monkey LOC101865873 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101865959 Cynomolgus monkey LOC101865959 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101866059 Cynomolgus monkey LOC101866059 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101866201 Cynomolgus monkey LOC101866201 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101866337 Cynomolgus monkey LOC101866337 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101866542 Cynomolgus monkey LOC101866542 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101866646 Cynomolgus monkey LOC101866646 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101866986 Cynomolgus monkey LOC101866986 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101867007 Cynomolgus monkey LOC101867007 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101867273 Cynomolgus monkey LOC101867273 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101867325 Cynomolgus monkey LOC101867325 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101867411 Cynomolgus monkey LOC101867411 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101925040 Cynomolgus monkey LOC101925040 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101925167 Cynomolgus monkey LOC101925167 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101925273 Cynomolgus monkey LOC101925273 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus
LOC101925363 Cynomolgus monkey LOC101925363 Gene cDNA Clone (full-length ORF Clo​小鼠睾丸畸胎瘤细胞；F9ne) cDNA Cynomolgus
LOC101925459 Cynomolgus monkey LOC101925459 Gene cDNA Clone (full-length ORF Clone) cDNA Cynomolgus