研选同类产品更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
三一造血
- 英文名：
Stemery Serum Peplacement
- 规格：
100mL/瓶/500mL/瓶
| 规格： | 100mL/瓶 | 产品价格： | ￥3600.0 |
|---|---|---|---|
| 规格： | 500mL/瓶 | 产品价格： | ￥14500.0 |
产品简介
本产品含有多种不同细胞生长因子，包括PDGF、VEGF、EGF等。可以完全替代胎牛血清（FBS）甚至于在支持一些细胞培养的效果上优于FBS。本产品能够支持包括间充质干细胞的增殖，成骨分化、成脂分化、造血干细胞以及诱导性多能干细胞的生长等。
产品特点
- 每批次产品均混均经过严格的安全性检控
- 无菌，无支原体，地内毒素
- 营养丰富，可支持包括MSC在内多种细胞的快速增殖
使用该产品引用文献
文献The transplantation of rapamycin-treated senescent human mesenchymal stem cells with enhanced proangiogenic activity promotes neovascularization and ischemic limb salvage in mice
三一造血（STEMERY）是一家由留学归国学者创办的致力血液细胞分离；培养及应用的国家高新技术企业。公司目前围绕血液细胞已经形成了一条特色产品服务于基础研究，药物开发以及细胞治疗工业等客户。1FLOSEP血液细胞分离技术2血液原代细胞库；3血液细胞培养产品； 4血液细胞改造防治肿瘤技术；5血液细胞体外大规模扩增（人工血液）技术。我们的目标是从细胞免疫学角度解决肿瘤，自身免疫性疾病以及血荒等难题。
公司自创办以来，先后获得过“人社部优秀留学人员创业项目” “福建省留学人员重点支持项目” “福州市优秀留学人员支持项目”“福州市五四青年奖章”“厦门市双百人才”“福建省高层人才”“中国创新创业大赛福建赛区二等奖”等荣誉称号。我们从一小小的梦想和行动起步，将脚踏实地把“小细胞助力大健康”的梦想变成现实，矢志不渝。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
用户评价
暂无用户评价
文献和实验该产品被引用文献
MATERIALS AND METHODS
Isolation of UCMSCs and culture conditions
Human umbilical cords (UCs; n = 5) were obtained from consenting patients who delivered full-term (38–40 weeks) infants by
cesarean section. The study was approved by the Ethics
Committee of Tongji Medical College, Huazhong University of
Science, and Technology and followed the principles of the
Declaration of Helsinki. The UCs were rinsed with phosphatebuffered saline (PBS; HyClone, Logan, UT, USA) to remove blood
cells. Subsequently, the samples were cut into 1 cm segments, and
the UC veins, arteries, and amnion were removed. The UCs were
then minced into 1–3 mm3 pieces. The minced pieces were placed
in a 55 cm2 culture dish and incubated in DMEM/F-12 (HyClone)
supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad,
CA, USA). After 2 weeks, the UC tissue was removed, and the
adherent cells were passaged with 0.25% trypsin (Gibco) for 1 min
at 37 °C. The obtained cells were centrifuged at 250 ×g for 5 min
and subcultured at a density of 4000 cells/cm2 in α-MEM (HyClone)
supplemented with 5% human platelet lysate (HPL; Stemery
Biotech, Fujian, China) and 100 U/mL penicillin/streptomycin
(Gibco). The concentration of HPL was determined by CCK-8
analysis (Supplementary Fig. S1). Cell passaging was performed
when the monolayer of adherent cells reached 70%–80%
confluence. All cells were maintained at 37 °C in a humidified
atmosphere with 5% CO2.
Isolation of UCMSCs and culture conditions
Human umbilical cords (UCs; n = 5) were obtained from consenting patients who delivered full-term (38–40 weeks) infants by
cesarean section. The study was approved by the Ethics
Committee of Tongji Medical College, Huazhong University of
Science, and Technology and followed the principles of the
Declaration of Helsinki. The UCs were rinsed with phosphatebuffered saline (PBS; HyClone, Logan, UT, USA) to remove blood
cells. Subsequently, the samples were cut into 1 cm segments, and
the UC veins, arteries, and amnion were removed. The UCs were
then minced into 1–3 mm3 pieces. The minced pieces were placed
in a 55 cm2 culture dish and incubated in DMEM/F-12 (HyClone)
supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad,
CA, USA). After 2 weeks, the UC tissue was removed, and the
adherent cells were passaged with 0.25% trypsin (Gibco) for 1 min
at 37 °C. The obtained cells were centrifuged at 250 ×g for 5 min
and subcultured at a density of 4000 cells/cm2 in α-MEM (HyClone)
supplemented with 5% human platelet lysate (HPL; Stemery
Biotech, Fujian, China) and 100 U/mL penicillin/streptomycin
(Gibco). The concentration of HPL was determined by CCK-8
analysis (Supplementary Fig. S1). Cell passaging was performed
when the monolayer of adherent cells reached 70%–80%
confluence. All cells were maintained at 37 °C in a humidified
atmosphere with 5% CO2.
技术资料需要更多技术资料 索取更多技术资料
研选
Stemery血清替代物RC-002
￥3600 - 14500
