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    研选Helios UltraGRO细胞营养添加物

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    Helios UltraGRO细胞营养添加物

    丁香通用户服务中心 查看详情

      • 货号:

        HPCPLCRL05/10/50

      • 供应商:

        Aventacell

      • 数量:

        100

      • 是否是肿瘤细胞:

      • 运输方式:

        干冰运输


       
      干细胞填充物

              UltraGRO™适用于多种人MSC的培养,如:骨髓间充质干细胞(BMMSC)、脂肪间充质干细胞(ADMSC)、脐带间充质干细胞(UCMSC)等。
              含有多重生长因子萃取物,提供干细胞完整平衡和自然的生长环境,不含血清不含其它动物来源蛋白质,不需额外添加胎牛血清(FBS)或重组生长因子。
              实验证实,5%UltraGRO™搭配一般基础培养基即可获得与20%FBS相同的细胞生长速度并仍维持干细胞的特性与分化能力。

               UltraGRO™来自美国先进制造技术,每批次UltraGRO™,皆经过MSC培养测试,批次间稳定性高。
              UltraGRO™满足使用者在干细胞临床发展时,对细胞培养基高质量、高安全性、高效率、可重复性的要求。

      UltraGRO™有效提升您的细胞培养工艺:

      • 无动物来源/无血清 细胞营养添加物
      • 天然蛋白质提供干细胞自然生长环境
      • 富含多种蛋白质/细胞激素/生长因子
      • 细胞倍增时间24小时
      • 用量仅5%,即可获得比 20% FBS更快生长速度
      • 节省时间及成本,减少细胞培养基花费、减少人工花费
      • 适用于初代培养及继代培养
       

      UltraGRO™培养人MSC,细胞形态如下:

      细胞形态
           该产品被引用的文献
      1、A fully-closed and automated hollow fiber bioreactor for clinical-grade manufacturing of human mesenchymal stem/stromal cells. Amanda Mizukami, Mario Soares de Abreu Neto, Francisco Moreira, Ana Fernandes-Platzgummer, Yi-Feng Huang, William Milligan, Joaquim M. S. Cabral, Claudia L. da Silva, Dimas T. Covas, Kamilla Swiech. Stem Cell Rev. 2018;14(1):141-143. doi: 10.1007/ s12015-017-9787-4.
      2、The corneal epitheliotrophic abilities of lyophilied powder form human platelet lysates. Lily Wei Chen, Chien-Jung Huang, Wen-Hui Tu, Chia-Ju Lu, Yi-Chen Sun, Szu-Yuan Lin, Wei-Li Chen. PLoS One. 2018;13(3):e0194345. doi: 10.1371/ journal.pone.0194345.
      3、Transplantation routes affect the efficacy of human umbilical cord mesenchymal stem cells in a rat GDM model. Dan Wu, Shan Zou, Haibin Chen, Xiaoyan Li, Yetao Xu, Qing Zuo, Yi Pan, Shi-Wen Jiang, Hung Huang Lizhou Sun. Clin Chim Acta. 2017;475:137-146. doi: 10.1016/j.cca.2017.10.013.
      4、Comparison of corneal epitheliotrophic capacities among human platelet lysates and other blood derivatives. Chein-Jung Huang, Yi-Chen Sun, Karen Christopher, Amy Shih-I Pai, Chia-Ju Lu, Fung-Rong Hu, Szu-Yuan Lin, Wei-Li Chen. PLoS One. 2017 Feb 2;12(2):e0171008. doi: 10.1371/journal.pone.0171008.
      5、Integrated culture platform based on a human platelet lysate supplement for the isolation and scalable manufacturing of umbilical cord matrix-derived mesenchymal stem/stromal cells. de Soure AM, Fernandes-Platzgummer A, Moreira F, Lilaia C, Liu SH, Ku CP, et al. J Tissue Eng Regen Med; 2017;11(5):1630-1640.
      6、Long-Term Expansion in Platelet Lysate Increases Growth of Peripheral Blood-Derived Endothelial-Colony Forming Cells and Their Growth Factor-Induced Sprouting Capacity. Tasev D, van Wijhe MH, Weijers EM, van Hinsbergh VWM, Koolwijk P. PLoS One. 2015;10(6):e0129935
      7、Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells. Hemeda H, Giebel B, Wagner W. Cytotherapy. 2014;16(2):170-80.
      8、Human platelet lysate is a feasible candidate to replace fetal calf serum as medium supplement for blood vascular and lymphatic endothelial cells. Cytotherapy. Hofbauer P, Riedl S, Witzeneder K, Hildner F, Wolbank S, Groeger M, et al. 2014;16(9):1238-44.
      9、Human Wharton’s Jelly Mesenchymal Stem Cells Plasticity Augments Scar-Free Skin Wound Healing with Hair Growth. Sabapathy V, Sundaram B, V M S, Mankuzhy P, Kumar S. PLoS One. 2014; 9(4):e93726.
      10、The effect of platelet lysate fibrinogen on the functionality of MSCs in immunotherapy. Copland IB, Garcia MA, Waller EK, Roback JD, Galipeau J. Biomaterials. 2013;34(32) : 7840-50.
      11、Human platelet lysate stimulates high-passage and senescent human multipotent mesenchymal stromal cell growth and rejuvenation in vitro. Griffiths S, Baraniak PR, Copland IB, Nerem RM, McDevitt TC. Cytotherapy. 2013; 15(12):1469-83.
      12、Pooled human platelet lysate versus fetal bovine serum-investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use. Trojahn Kølle SF, Oliveri RS, Glovinski PV, Kirchhoff M, Mathiasen AB, Elberg JJ, et al. Cytotherapy. 2013; 15(9):1086-97.
      13、Manufacturing mesenchymal stromal cells for phase I clinical trials. Hanley PJ, Mei Z, da Graca Cabreira-Hansen M, Klis M, Li W, Zhao Y, Durett AG, Zheng X, Wang Y, Gee AP, Horwitz EM. Cytotherapy. 2013;15(4):416-22.
      14、Human platelets lysate as a promising growth-stimulating additive for culturing of stem cells and other cell types. Shanskii YD, Sergeeva NS, Sviridova IK, Kirakozov MS, Kirsanova VA, Akhmedova SA, et al. Bull Exp Biol Med. 2013; 156(1):146-51.
      15、Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications. Naaijkens BA, Niessen HW, Prins HJ, Krijnen PA, Kokhuis TJ, de Jong N, et al. Cell Tissue Res. 2012; 348(1):119-30.
      16、Platelet lysate from whole blood-derived pooled platelet concentrates and apheresis- derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components. Fekete N, Gadelorge M, Fürst D, Maurer C, Dausend J, Fleury-Cappellesso S, et al. Cytotherapy. 2012; 14(5):540-54.
      17、Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum. Ben Azouna N, Jenhani F, Regaya Z, Berraeis L, Ben Othman T, Ducrocq E, et al. Stem Cell Research & Therapy. 2012, 3(1):6.
      18、Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications. Govindasamy V, Ronald VS, Abdullah AN, Ganesan Nathan KR, Aziz ZA, Abdullah M, et al. Cytotherapy. 2011;13(10):1221-33.
      19、Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. Rauch C, Feifel E, Amann EM, Spötl HP, Schennach H, Pfaller W, et al. ALTEX. 2011; 28(4) : 305-16.
      20、Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. Crespo-Diaz R, Behfar A, Butler GW, Padley DJ, Sarr MG, Bartunek J, Dietz AB, Terzic A. Cell Transplant. 2011; 20(6):797-811.
      21、Influence of Platelet Lysate on the Recovery and Metabolic Performance of Cryopreserved Human Hepatocytes Upon Thawing. Tolosa L, Bonora-Centelles A, Donato MT, Mirabet V, Pareja E, Neg-ro A, et al. Transplantation. 2011; 91(12):1340-6.
      22、Platelet-lysate-Expanded Mesenchymal Stromal Cells as a Salvage Therapy for Severe Resistant Graft-versus-Host Disease in a Pediatric Population. Lucchini G, Introna M, Dander E, Rovelli A, Balduzzi A, Bonanomi S, et al. Biol Blood Marrow Transplant. 2010; 16(9):1293-301.
      23、Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors. Avanzini MA, Bernardo ME, Cometa AM, Perotti C, Zaffaroni N, Novara F, et al. Haematologica. 2009;94(12):1649-60.
      24、Treatment of refractory acute GVHD with third-party MSC expanded in platelet lysate-containing medium. von Bonin M, Stölzel F, Goedecke A, Richter K, Wuschek N, Hölig K, et al. Bone Marrow Transplant. 2009; 43(3):245-51.
      25、Platelet lysate stimulates wound repair of HaCaT keratinocytes. Ranzato E, Patrone M, Mazzucco L, Burlando B. Br J Dermatol. 2008;159(3):537-45.
      26、Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts. Capelli C, Domenghini M, Borleri G, Bellavita P, Poma R, Carobbio A, et al. Bone Marrow Transplant. 2007; 40, 785–791.
      27、Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. Doucet C, Ernou I, Zhang Y, Llense JR, Begot L, Holy X, et al. J Cell Physiol. 2005; 205(2):228-36.

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      资料下载:

      UltraGROTM--使用方针、保存方法及注意事项.doc 附件下载 (下载 7 次)

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    代理商
    8 年  VIP 供应商

    江苏普诺生生物科技有限公司

    • 联系人:

      陆小姐

    • 所在地区:

      江阴市东盛西路6号,江苏,中国

    • 业务范围:

      试剂,技术服务,细胞库 / 细胞培养,ELISA 试剂盒

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