端粒&端粒酶PCR芯片 Telomeres & Telome

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Telomeres & Telomerase PCR Array

端粒&端粒酶PCR芯片

Product	Species	Technology	Cat. No.
Telomeres & Telomerase PCR Array	Human	Gene Expression	PAHS-010Z
Telomeres & Telomerase PCR Array	Mouse	Gene Expression	PAMM-010Z
Telomeres & Telomerase PCR Array	Rat	Gene Expression	PARN-010Z

Telomeres & Telomerase RT² Profiler™ PCR Array profiles the expression of 84 key genes central to telomere replication and maintenance. Telomeres, repetitive DNA regions of hexanucleotide repeats, protect chromosomal ends from deterioration during DNA replication. Telomerase (TERT), a reverse transcriptase, forms a complex with an RNA template and cofactors to extend telomeres. The shelterin protein complex then binds the 3' single-stranded end of telomeric DNA, protecting it from DNA damage responses. Inhibition of this process leads to short telomeres and premature aging-related diseases, whereas uncontrolled telomere lengthening promotes carcinogenesis. Research into telomeres often utilizes simpler model organisms, such as yeast, meaning that many mechanistic details have yet to be discovered or confirmed in mammalian systems by you. This array contains genes involved in telomere maintenance as well as genes comprising the telomerase and shelterin complexes and their regulators. This array also includes genes recently described in telomere regulation, such as the SLX4 complex, as well as other genes associated with telomeres but with poorly understood functions. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes involved in telomere maintenance and genome integrity with this array.

端粒&端粒酶PCR芯片可用于研究端粒复制和维持的84个核心基因的表达。端粒，染色体末端的六碱基重复DNA片段，在DNA复制过程中保护染色体末端免于融合和退化。端粒酶，一个反转录酶，与RNA模板和辅助因子形成复合体进行端粒延伸，该蛋白复合体与端粒DNA的
3’单链末端结合后将保护其免受DNA损伤应激作用。端粒修复活性的抑制会导致端粒的缩短，以至老化相关的疾病提前到来，反之，端粒延伸活性的失控则会促进癌症的发生。端粒的研究一般是利用简单的模式生物如酵母来进行的，意味着在哺乳动物中的具体机制细节需要得到进一步的发掘和验证。该芯片涵盖了端粒维持相关的基因以及端粒酶、端粒酶复合物和对应的调节因子基因，同时也包含了近期研究发现参与端粒调节的基因（如SLX4复合体），以及与端粒相关但功能尚不明确的基因。通过实时定量PCR，研究者可以简单可靠地同时分析端粒维持和基因组完整性的相关基因的表达。
Telomere Maintenance:ACD, BLM, DCLRE1C, DKC1, ERCC1, ERCC4, HSPA1L, MRE11A, MYC, NBN (NBS1), PARP1 (ADPRT1), PIF1, POT1, PRKDC, PTGES3, RAD50, RFC1, RTEL1, SMG6, TEP1, TERF1, TERF2, TERF2IP, TERT, TINF2, TNKS (TIN1), TNKS2, XRCC5, XRCC6 (G22P1).
Telomere-Associated Complexes:
Shelterin:ACD, POT1, RAP1A, TERF1, TERF2, TINF2.
Telomerase:DKC1, GAR1, NHP2, NOP10, TERT, WRAP53.
SLX4:SLX4, ERCC1, ERCC4, EME1, MSH2, MSH3, MUS81, PLK1, TERF2, TERF2IP.
Telomere Regulation:
Regulation of Telomere Length:RTEL1, TEP1, TERF1, TERF2IP, TNKS (TIN1).
Shelterin Complex Regulation:DCLRE1B, KRIT1, RASSF1, RAPGEF1, TPP1.
Telomerase Regulation:ABL1, AKT1, ATP5C1, BCL2, EGF, IGF1, KRAS, MEN1, MYC, PAX8, PINX1, PPARG, PPP2R1A, PPP2R1B, PRKCA, PRKCB, RB1, SART1, SMAD3, SP1, SSB, TGFB1, TP53.
Other Genes Associated with Telomeres:ATM, CDK2, CHEK1, CHEK2 (RAD53), HAT1, HNRNPA2B1, HNRNPD, HSP90AA1, NCL, OBFC1, PURA, RAD17, RIF1, SIRT2, SIRT6, TP53BP1, SUN1