视黄酸信号通路PCR芯片 Retinoic Acid Sig

“英拜为您实验加速”
技术服务网址：http://www.yingbio.com/
服务热线：400-696-6643、 18019265738
邮箱：daihp@yingbio.com 、 huizhang1228@foxmail.com

Retinoic Acid Signaling PCR Array

视黄酸信号通路PCR芯片

Product	Species	Technology	Cat. No.
Retinoic Acid Signaling PCR Array	Human	Gene Expression	PAHS-180Z
Retinoic Acid Signaling PCR Array	Mouse	Gene Expression	PAMM-180Z
Retinoic Acid Signaling PCR Array	Rat	Gene Expression	PARN-180Z

The Human Retinoic Acid Pathway RT² Profiler PCR Array profiles the expression of 84 key genes involved in retinoic acid signaling. Retinoic acid (RA) is the primary functional derivative of vitamin A (retinol) and its activity is implicated in many aspects of vertebrate development and homeostasis, while disruptions in this pathway cause developmental abnormalities and disrupt function in adipose, cardiac, nervous, reproductive, and integumentary tissues, among others. RA acts primarily by binding a family of nuclear receptors (the retinoic acid receptors alpha, beta, and gamma) that then heterodimerize with their partners (the retinoid X receptors alpha, beta, and gamma) and alter transcription. Additionally, recent evidence suggests that an alternative receptor (PPAR delta) also responds to RA signaling in some tissues and that retinol, and RA may also induce non-genomic cellular effects. Thus, the effect of RA depends on the expression of its cognate receptors, enzymes responsible for converting retinol to RA, metabolic enzymes that reduce RA levels, and transport proteins, some of which act as signal transducers that appear to influence the distribution of RA and its metabolic precursors. This array includes genes encoding the known receptors and proteins responsible for synthesis, transport and metabolism of RA and its precursors, as well as downstream targets of RA signaling. Results obtained with this array can yield insights into RA signaling mechanisms and responses. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in retinoic acid signaling with this array.
The RT² Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available.
人类的视黄酸信号通路PCR芯片用于研究参与视黄酸信号通路的84关键基因的表达。视黄酸(RA)具有重要的生物学功能，由维生素A(视黄醇)派生而来，其活性涉及多种生物学过程如脊椎动物发育和内稳态,而中断这个途径会导致心脏、神经、生殖、和皮肤组织等发育异常和功能中断。RA通过绑定其家族的核心受体(视黄酸受体α、β和γ),然后二聚化和伴侣(视黄素X受体α、β和γ)和改变转录，发挥重要功能。此外,最近的证据表明,另一个受体(PPARδ) 在某些组织和视黄醇中也对RA信号产生应答，RA也可能诱发非基因组细胞的效应。因此RA的影响程度取决于其同源受体、负责转换视黄醇为RA的酶、降低RA水平的代谢酶和运输蛋白等的表达,其中一些作为传感器信号，影响RA的分布和前体代谢。这个芯片包含编码已知受体和负责合成的蛋白、运输和RA代谢蛋白及其前体，以及RA信号下游的目标蛋白的基因。结果可进一步了解RA信号机制和响应。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地分析人类视黄酸信号通路相关基因的表达。
PCR芯片仅用于分子生物学应用。本产品不用于疾病的诊断、预防和治疗。
提供96孔板——384 -(4 x 96)板,和100孔板

Retinoic Acid & Retinol Receptors:RARA, RARB, RARG, RXRA, RXRB, RXRG, PPARD, STRA6.
Retinoic Acid & Retinol Metabolism:ADH1A, ALDH1A1, ALDH1A2, ALDH1A3, CYP1B1, CYP26A1, CYP26B1, CYP26C1, DHRS3, DHRS9, LRAT, RDH10.
Retinoic Acid & Retinol Transporters:CRABP1, CRABP2, FABP5, RBP1, RBP2, RBP4, STRA6.
Non-Canonical Retinoic Acid Signal Transduction:CRABP1, FABP5, LRAT, PPARD, PPARG, RARA, STRA6.
Retinoic Acid Receptor Targets:ADH1A, APOA2, BHLHE40, CD38, CDX1, CRABP1, CRABP2, CYP26A1, CYP26B1, CYP26C1, DHRS9, EFNB1, EGR1, EPO, FOXA1, GBX2, HNF1B, HOXA1, HOXA5, HOXB1, HOXB4, HSD17B2, KLF4, LEFTY1, MEIS2, MYC, PLAT, RARA, RARB, RARG, RARRES3, RBP1, RBP4, SHH, SREBF1, STRA6, STRA8, TFAP2C, TGM2, UCP1, WNT8A.
Retinoic Acid Pathway Regulators:CHD7, HOXA1, MEIS2, NRIP1, PAX6, PPARG, TBX1.
Signal Transduction: BMP2, CD38, DCX, EFNB1, EPO, FGF8, JAG1, KLF4, LEFTY1, PLAT, RARRES3, RET, SHH, TGFB2, WNT5A, WNT8A.
Transcription Factors & Regulators:ASCL1, BHLHE40, CDX1, CHD7, DLX5, EGR1, FOXA1, FOXG1, GATA4, GBX2, GLI1, HNF1B, HOXA1, HOXA5, HOXB1, HOXB4, ISL1, KLF4, LHX1, MAFB, MEIS2, MSX2, MYC, NANOG, NEUROD1, NRIP1, OLIG2, OTX2, PAX6, PITX2, PPARA, PPARD, PPARG, RARA, RARB, RARG, RXRA, RXRB, RXRG, SOX2, SOX9, SREBF1, TBX1, TFAP2C.
Development:
Adipogenesis:EGR1, FABP5, FOXA1, KLF4, MSX2, NRIP1, PPARA, PPARD, PPARG, RBP4, SREBF1, STRA6, UCP1.
Cardiovascular System Development:BMP2, CHD7, DHRS3, DLX5, EPO, FGF8, GATA4, ISL1, JAG1, PITX2, SHH, SOX9, STRA6, TBX1, TGFB2, WNT5A.
Body Pattern Formation:BMP2, CDX1, EFNB1, FGF8, FOXA1, FOXG1, GATA4, GBX2, GLI1, HOXA1, HOXA5, HOXB1, HOXB4, HNF1B, JAG1, LEFTY1, LHX1, MAFB, MEIS2, NANOG, NEUROD1, OTX2, PITX2, SHH, TBX1, WNT5A, WNT8A.
Neurological Development:ASCL1, BMP2, CHD7, DCX, DLX5, EFNB1, FGF8, FOXA1, FOXG1, GBX2, HNF1B, HOXA1, HOXB1, ISL1, MAFB, MEIS2, NEUROD1, OLIG2, OTX2, PAX6, PITX2, RET, SHH, SOX2, STRA6, TBX1, TFAP2C, TGFB2, TUBB3, WNT5A.
Sex Determination & Development:CHD7, FGF8, GATA4, GLI1, HNF1B, LHX1, MAFB, RBP4, SHH, SOX2, SOX9, STRA6, STRA8, WNT5A.
Skin Development & Homeostasis:KLF4, LRAT, PAX6, PPARA, PPARD, PPARG, SHH, TFAP2C, MSX2.
Stem Cell Development & Maintenance:GLI1, KLF4, NANOG, SHH, SOX2, TFAP2C, WNT5A.