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- E2039-10UN
- 2025年09月19日
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- 详细信息
- 技术资料
- 保存条件：
软骨素酶AC
- 英文名：
Chondroitinase AC from Flavobacterium heparinum
- 库存：
软骨素酶AC
- 供应商：
研卉生物
- CAS号：
9047-57-8
- 规格：
10UN
Chondroitinase AC from Flavobacterium heparinum
recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis
Unit Definition
1 unit is defined as the amount of enzyme that will liberate 1.0 μmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The εμΜ for the reaction product Δ-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for Δ-Di-6S (chondroitin sulfate C).
Application
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
应用
单位定义
性质
| Related Categories | Application Index, Chondroitin and Chondroitinases, Chondroitinase AC, Chondroitinase AC and Disaccharide Standards, Clinical Chemistry, |
| recombinant | expressed in E. coli |
| assay | ≥90% (SDS-PAGE) |
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