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文献和实验Construction and Characterization of Adenovirus Vectors
. To prepare 293N3S cells in suspension: v. Transfer six 150-mm plates of confluent 293N3S cells to a 3-L spinner flask. Bring the total volume in the flask to 1 L with 1X maintenance medium.
culture hood. Ensure that any alcohols and wrapping paper are kept clear of the bunsen burner. Pre-sterilize the hood before use, and use disposable sterile gloves. Loosen the cap of a tissue culture flask and the cap of a stock bottle of tissue culture
Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
Basic Protocol 1: Differentiating hESC to CM on Tosoh 10 Microcarriers in Ultra‐Low Attachment Plates under Static Conditions Basic Protocol 2: Differentiating hESC to CM on Microcarriers in Spinner Flask
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