CD79A Antibody

3351:

Immunofluorescence , Western Blotting

CD79A Antibody detects endogenous levels of total CD79A protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn217 of human CD79A. Antibodies are purified by peptide affinity chromatography

Western Blotting

Western blot analysis of extracts from Raji and Daudi cell lines using CD79A Antibody.

IF-IC

Confocal immunofluorescent analysis of Ramos (left) and Jurkat cells (right) using CD79A Antibody (green). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye).

Antigen receptors found on the surface of B-cells contain a hetrodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B-cell development and function (3). Together these two proteins and the associated B cell receptor initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) is found in the CD79A intracellular region appears to be is important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). CD79A is a marker for B-lineage lymphoblastic leukemia (8); mutations in the corresponding mb-1 gene are responsible for abnormally low levels of functional B cell receptors in some cases of chronic B-cell lymphocytic leukemia (9).

1. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
2. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
4. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
5. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
6. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
7. Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
8. Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
9. Vuillier, F. et al. (2005) Blood 105, 2933-40.

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For Research Use Only. Not For Use In Diagnostic Procedures.