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- Cell Signaling Technology
- 3419
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- 2025年09月11日
- IP
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Streptavidin (Sepharose Bead Conjugate)
- 应用范围：
IP
- 适应物种：
All
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 标记物：
Sepharose Bead
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
400 ul (40 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul (40 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
Streptavidin (Sepharose Bead Conjugate) #3419
Protocols
- 3419:
- Immunoprecipitation
Description
Streptavidin (Sepharose Bead Conjugate) is useful for the precipitation of biotinylated proteins (1,2). Recombinant streptavidin is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads.
IP
Immunoprecipitation of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein using Myc-Tag (9B11) Mouse mAb (Biotinylated) #2084 and Immobilized Streptavidin (Bead Conjugate) (Lane 1) shows immunocomplexes pulled down using Streptavidin (Sepharose Bead Conjugate). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using Myc-Tag (9B11) Mouse mAb (HRP Conjugate) #2040.
IP
Immunoprecipitation of extracts from NIH/3T3 cells using PTEN (138G6) Rabbit mAb (Biotinylated) #9583 and Streptavidin (Sepharose Bead Conjugate) (Lane 1). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using PTEN (26H9) Mouse mAb #9556.
IP
Immunoprecipitation of extracts from Jurkat cells using Phospho-Akt (Thr308) (244F9) Rabbit mAb (Biotinylated) #3454 and Streptavidin (Sepharose Bead Conjugate) (Lane 1). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106.
Directions for Use
Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml pre-incubated with biotinylated primary antibody. See protocol for more details.
Background
Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).
- Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95.
- Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72.
- Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5.
- Reznik, G.O. et al. (1998) Proc Natl Acad Sci USA 95, 13525-13530.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2908 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Biotinylated)
- 2912 GFP (D5.1) XP® Rabbit mAb (Biotinylated)
- 2995 Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb (Biotinylated)
- 3642 Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated)
- 9583 PTEN (138G6) Rabbit mAb (Biotinylated)
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7072 Phototope® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验【原创】Protein A Sepharose 之进化篇。。。
这两种蛋白质A柱纯化的结果。 待纯化样品：人血清 实验材料： GE公司的rProtein A Sepharose FF（E、D、A、B、C结构域串联） Putus公司的rProtein A Sepharose FF（3个B结构域串联） 实验方法： 分别按照每个公司的说明书来操作，洗脱条件分别为pH值3.0和4.5， SDS-PAGE检测结果如下：。 Lane 1 Marker [img][/img]Protein A 柱之进化 Protein A是一种金黄色葡萄球菌细胞壁蛋白
相关专题 (一) 原理 葡萄球菌A蛋白(SPA)具有与多种哺乳动物 IgG分子Fc段结合的能力，并与不同IgG亚类的结合力有所差别。利用改变PH及离子强度可洗脱结合于SPA-Sepharose CL-4B柱上的IgG或不同的IgG亚类，可直接纯化血清或小鼠腹水中的IgG抗体。 (二) 操作步骤 用0.1M PH8.0 磷酸缓冲液 浸泡SPA-Sepharose CL-4B凝胶，15min。按1g干胶200ml
Polysaccharicb-Conjugate Vaccines
It was recognized early this century that small molecules, called haptens, can be made immunogenic after conjugation to carrier proteins (1 ), This principle was thereafter applied successfully to improve the rmmunogenicity of (poly
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