- 询价
- Cell Signaling Technology
- 9099
- USA
- 2025年09月11日
- W
- H,M,R,Mk,Dm,B,Dg
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
α-Tubulin (11H10) Rabbit mAb (HRP Conjugate)
- 抗原：
synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin protein
- 应用范围：
W
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Dm,B,Dg
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 标记物：
HRP
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk Dm B (Dg) | Endogenous | 52 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | α-Tubulin (11H10) Rabbit mAb (HRP Conjugate) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin protein. Western Blotting
Western blot analysis of extracts from various cell lines using α-Tubulin (11H10) Rabbit mAb (HRP Conjugate). |
| Description | This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (α-Tubulin (11H10) Rabbit mAb #2125). |
| Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验家兔（Rabbit,Oryctolagus curiculus）
家兔（Rabbit,Oryctolagus curiculus） 一、生物学特性和解剖生理特点 1．家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。 2．家兔体小力弱、胆小怕惊、怕热、怕潮，喜欢安静、清洁、干燥、凉爽的环境，不能忍受污秽的条件。 3．是食草类（Herbivorous）单胃动物。饲养原则是以青粗食米为主，精饲料为辅。喜欢独居，白天活动少，都处于假眠或休息状态，夜间活动大，吃食多。有啃木、扒土的习惯
盐水。 （6）PH7.8饱和硫酸铵溶液及半饱和硫酸铵溶液。 （7）萘氏试剂及聚乙二醇(PEG，MW2000)。 （8）纯化的特异性抗体或抗Ig抗体。 （9）HRP(RZ>3.0)。 （10）Sephadex G-25层析柱(2cm×50cm)。 （11）搅拌器，分光光度计，离心机。 （12）透析袋，大、小烧杯，试管，吸管等。 二、简易过碘酸钠法 本法是以NaIO4先将HRP表面的糖分子氧化成醛基，然后再与Ig上的氨基相结合，所获酶标记抗体的产率高，将近70%的HRP和Ig结合，99%的Ig
Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine? and psoralen?conjugated oligonucleotides, and include attachment of the linker, preparation
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