- 询价
- Cell Signaling Technology
- 5346
- USA
- 2026年05月28日
- W
- H,M,R,Mk,Z,B,C
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)
- 抗原：
synthetic peptide corresponding to the amino terminus of human β-tubulin
- 应用范围：
W
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,M,R,Mk,Z,B,C
- 级别：
详见MSDS文件
- 标记物：
HRP
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Z=Zebrafish B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk Z B (C) | Endogenous | 55 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) detects endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human β-tubulin. Western Blotting
Western blot analysis of extracts from various cell lines using β-Tubulin (9F3) Rabbit mAb (HRP Conjugate). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (β-Tubulin (9F3) Rabbit mAb #2128). |
| Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
实验助攻 | WB 实验 (Western Blot) 杂带太多？原因就这几个！
，并不会与重组 β-tubulin 蛋白发生交叉反应。 c-Myc 抗体 (YA497) www.medchemexpress.cn/antibody/c-myc-rabbit-mab.html 该抗体可检测 c-Myc 总蛋白的内源水平。 HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L www.medchemexpress.cn/antibody/peroxidase-conjugated-affinipure-goat-anti-mouse
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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