- 询价
- Cell Signaling Technology
- 5292
- USA
- 2025年10月10日
- IHC-P, IF-IC, F
- All
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
BrdU (Bu20a) Mouse mAb
- 抗原：
BrdU conjugated to BSA
- 应用范围：
IHC-P, IF-IC, F
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
All
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (100 assays)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (100 assays) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IHC-P IF-IC F | All | Endogenous | Mouse IgG1 |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | BrdU (Bu20a) Mouse mAb detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with BrdU conjugated to BSA. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HeLa cells, without (left) and with (right) BrdU incorporation, using BrdU (Bu20a) Mouse mAb. Flow Cytometry
Flow cytometric analysis of Jurkat cells incorporated with BrdU (30 minutes), using BrdU (Bu20a) Mouse mAb versus propidium iodide (DNA content). Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or BrdU incorporated for 30 minutes (green), using BrdU (Bu20a) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells using BrdU (Bu20a) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications (1-4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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