- 询价
- Cell Signaling Technology
- 4710
- USA
- 2026年11月01日
- W, IP, IHC-P, IF-IC
- H,M,R
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- 文献和实验
- 技术资料
- 抗体英文名：
TRAF1 (1F3) Rat mAb
- 抗原：
TRAF1-GST fusion protein and purified by protein G affinity chromatography
- 应用范围：
W, IP, IHC-P, IF-IC
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R | Endogenous | 50 | Rat IgG2a |
| Protocols | |
|---|---|
| Specificity / Sensitivity | TRAF1 (1F3) Rat mAb detects endogenous levels of total TRAF1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a TRAF1-GST fusion protein and purified by protein G affinity chromatography. Western Blotting
Western blot analysis of extracts from Raji and SR cells, using TRAF1 (1F3) Rat mAb. IHC-P (paraffin)
Immunohisotchemical analysis of paraffin-embedded human tonsil using TRAF1 (1F3) Rat mAb. IF-IC
Immunofluorescent staining of Raji cells showing cytoplasmic staining, using TRAF1 (1F3) Rat mAb. |
| Background | TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
mAb. It is important to verify that no secondary reagent will bind the BD Fc Block™. Please see description in "The Uses of BD Fc Block™ in Immunophenotyping of Mouse and Rat Leukocytes". For very low-density cell surface markers (e.g.,cytokine
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
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