Cyclin B1 Antib

4138:

Western Blotting

Cyclin B1 Antibody detects endogenous levels of total cyclin B1.

Polyclonal antibodies are produced by immunizing animals with a syntheic peptide corresponding to residues near the amino terminus of human cyclin B1. Antibodies are purified using peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell types using Cyclin B1 Antibody.

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Cyclin B1 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) showing concentrated cyclin B1 staining surrounding mitotic chromatin.

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13).

1. Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
2. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
3. Watanabe, N. et al. (1995) EMBO J. 14, 1878-1891.
4. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
5. Hunter, T. et al. (1995) Cell 80, 225-236.
6. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.
7. McGowan, C.H. et al. (1993) EMBO J. 12, 75-85.
8. Izumi, T. et al. (1991) Mol. Cell. Biol. 11, 3860-3867.
9. Li, J. et al. (1995) Mol. Biol. Cell 6, 1111-1124.
10. Li, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 502-507.
11. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-220.
12. Peter, M. et al. (2002) EMBO Rep. 3, 551-556.
13. Jackman, M. et al. (2003) Nat. Cell Biol. 5, 143-148.

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• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.