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α-Tubulin (11H10) Rabbit mAb (

Biotinylated)
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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年09月08日
  • W
  • H,M,R,Mk,Dm,B,Dg
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      α-Tubulin (11H10) Rabbit mAb (Biotinylated)

    • 抗原

      synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin

    • 应用范围

      W

    • 供应商

      CST

    • 保质期

      详见说明书

    • 级别

      详见MSDS文件

    • 库存

      大量

    • 适应物种

      H,M,R,Mk,Dm,B,Dg

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  B=Bovine  Dg=Dog
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W H M R Mk Dm B (Dg) Endogenous 52 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    α-Tubulin (11H10) Rabbit mAb (Biotinylated) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines using α-Tubulin (11H10) Rabbit mAb (Biotinylated) and developed using Streptavidin-HRP #3999.

    Description

    This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.

    Background

    The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

    1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938 -947.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    Rabbit monoclonal antibody is produced�under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.


    For Research Use Only. Not For Use In Diagnostic Procedures.

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