- 询价
- Cell Signaling Technology
- 2306
- USA
- 2025年11月14日
- W, IP, IHC-P, IF-F
- M,R,H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
DARPP-32 (19A3) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Glu160 of human DARPP-32
- 应用范围：
W, IP, IHC-P, IF-F
- 适应物种：
M,R,H
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-F | M R (H) | Endogenous | 32 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | DARPP-32 (19A3) Rabbit mAb detects endogenous levels of totoal DARPP-32 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu160 of human DARPP-32. Western Blotting
Western blot analysis of extracts from rat and mouse brain using DARPP-32 (19A3) Rabbit mAb. Western Blotting
Western blot analysis of extracts from rat, mouse brain, wild-type mouse striatum and DARPP-32 knock-out mouse striatum, using DARPP-32 (19A3) Rabbit mAb (wild-type mouse striatum and DARPP-32 knock-out mouse striatum was kindly provided by Dr. Paul Greengard and Helen Bateup, Rockefeller University, New York, NY). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain using DARPP-32 (19A3) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat retina using DARPP-32 (19A3) Rabbit mAb. IF-F
Confocal immunofluorescent analysis of normal rat cerebellum, retina, and ciliary epithelium using DARPP-32 (19A3) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In Vivo
32 and 34 ). The creation of these recombinant constructs is laborious and requires specialized techniques, however. A technique that can efficiently deliver simple plasmids containing the gene of interest in vivo allows the screening of gene
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
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