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c-Rel Antibody
synthetic peptide corresponding to residues near the carboxy terminus of human c-Rel
W, IP, IHC-P, IF-IC, F
Rabbit
详见说明书
详见MSDS文件
CST
大量
H,Mk
2
-20°c
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
规格: | 产品价格: | ¥请询价 | |
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规格: | 40 ul (4 western blots) | 产品价格: | ¥请询价 |
规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Source |
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W IP IHC-P IF-IC F | H Mk | Endogenous | 78 | Rabbit |
Protocols | |
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Specificity / Sensitivity | c-Rel Antibody detects endogenous levels of total c-Rel protein. Cross reactivity was not detected with other family members. |
Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human c-Rel. Antibodies were purified by protein A and peptide affinity chromatography. Western BlottingWestern blot analysis of extracts from K562, Raji and 293 cells, using c-Rel Antibody. IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using c-Rel Antibody in the presence of control peptide (left) or antigen-specific peptide (right). IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded Hodgkin's lymphoma, showing nuclear and cytoplasmic localization using c-Rel Antibody. IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded human skin, using c-Rel Antibody. Flow CytometryFlow cytometric analysis of K562 cells using c-Rel Antibody (blue) compared to a nonspecific negative control antibody (red). IF-ICConfocal immunofluorescent analysis of HeLa cells, untreated (left) or TNFα-treated (#2169, 20 ng/ml for 20 min, right), using c-Rel Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). |
Background | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
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Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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