- 询价
- Cell Signaling Technology
- 3944
- USA
- 2025年09月07日
- W, F
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated)
- 抗原：
recombinant fusion protein corresponding to full-length human S6 ribosomal protein
- 应用范围：
W, F
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | H M R Mk | Endogenous | 32 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | S6 Ribosomal Protein (54D2) Mouse mAb (Biotinlyated) detects endogenous levels of total S6 ribosomal protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein. Western Blotting
Western Blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells using S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated). Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, using S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated) (blue) compared to concentration matched Mouse mAb (MOPC-21) IgG1 Isotype control (Biotinylated) #4097 (red). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested in-house by Western Blot and flow cytometric analysis. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (S6 Ribosomal Protein (54D2) Mouse mAb #2317). |
| Background | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
, where the “xxx” represents the bead region) Bead coupling buffer (BCB; see recipe ) Biotinylated RGS protein (see protocol
Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors
the automated methodology we have employed for the successful detection of phosphorylation of S6 ribosomal protein (S6-RP) and the retinoblastoma protein (Rb) in response to PDGF stimulation in a mouse model of glial brain tumor development.
The Extracellular Signal-Regulated Kinase (ERK) Cascade in Neuronal Cell Signaling
of both the ribosomal protein S6 and of the histone H3 is completely prevented by a chemical inhibition of the ERK pathway, this ex-vivo system can be exploited in the future to investigate the regulation of the ERK cascade using slices from LV-injected
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